Quantitative studies on the bactericidal actions of serum and complement. I. A rapid photometric growth assay for bactericidal activity.

Effects of propolis, a honeybee product, on growth performance and immune responses of Barbus barbulus

Background: Propolis, a honeybee product, has been used empirically for centuries and was always mentioned as an immunomodulatory. OBJECTIVES: In this study, the effects of propolis on growth indices, innate immune responses and haematological parameters of Barbus barbulus were investigated. METHODS: Three hundred and sixty juvenile Barbus barbulus, weighing 102±8.2g were randomly divided int...

Quantitative studies on the bactericidal actions of serum and complement. II. Some implications for the mechanism of the bactericidal reaction.

Evaluation of Pasteurella multocida serotype B:2 resistance to immune serum and complement system

Members of gram-negative bacteria family Pasteurellaceae, include a large number of important economically human and veterinary pathogens. Organisms belonging to the family can colonize in mucosal surfaces of the respiratory, alimentary, genital tracts and cause diseases in various mammals, birds, and reptiles. Hemorrhagic septicemia is an acute disease of cattle and buffaloes in tropi...

The Stability of Complement-Mediated Bactericidal Activity in Human Serum against Salmonella

The complement cascade includes heat-labile proteins and care is required when handling serum in order to preserve its functional integrity. We have previously used a whole human serum bactericidal assay to show that antibody and an intact complement system are required in blood for killing of invasive isolates of Salmonella. The aim of the present study was to evaluate the conditions under whi...

Development of a high-throughput method to evaluate serum bactericidal activity using bacterial ATP measurement as survival readout

Serum Bactericidal Activity (SBA) assay is the method of choice to evaluate the complement-mediated functional activity of both infection- and vaccine-induced antibodies. To perform a typical SBA assay, serial dilutions of sera are incubated with target bacterial strains and complement. The conventional SBA assay is based on plating on agar the SBA reaction mix and counting the surviving bacter...